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. 2009 Oct 16;5(10):e1000628. doi: 10.1371/journal.ppat.1000628

Figure 2. Syt V-GFP is excluded from L. donovani promastigote-containing phagosomes.

Figure 2

Syt V-GFP RAW 264.7 cells were infected with either WT lpg1-KO, lpg2-KO, lpg2-KO+LPG2 L. donovani promastigotes or zymosan for 2 h, fixed and stained for DNA. A, Confocal images illustrating the presence of Syt V-GFP on parasite-containing phagosomes (shown by arrowheads). Presence (B) and relative levels (C) of Syt V-GFP were determined. Recruitment was determined on at least 100 phagosomes for each condition and expressed as a percentage of recruitment, and relative levels were determined by the P/C ratio as described in Materials and Methods . Three independent experiments were performed and the bars show the standard deviations of one representative triplicate (*, p≤0.05; **, p≤0.005; B and C, p values compare the presence and the accumulation of Syt V-GFP on phagosomes containing WT and lpg2-KO+LPG2 vs lpg1-KO and lpg2-KO parasites). Bar, 3 µm.