Figure 2.

Hyperspectral imaging represents a powerful technique for analysis of multiple QD-labeled biomarkers within a single specimen. While standard RGB camera cannot distinguish spectrally overlapping probes and is limited to analysis of few biomarkers, hyperspectral imaging applies narrow band-pass filters and takes a series of images for each wavelength, thus providing spectral information for each pixel of an image. Further application of spectral library allows accurate unmixing of individual spectrally-distinct components, enhancing the ability for molecular profiling [3].