Figure 6.

Detection of anion exchanger and ECA transcripts. A, Single-cell (nested) PCR demonstrated abundant AE3 message in individual, acutely dissociated hippocampal pyramidal neurons (area cornu ammon 1). B, Real-time PCR of hippocampal neuronal cultures showed absence of AE1, and an approximately fivefold predominance of AE3 over AE2 transcripts. C, CA14 transcripts were increased in hippocampus from knock-out (KO) mice. There was no significant difference in CA4 transcripts in the same tissues. *p < 0.05; ^nsp > 0.05. Error bars indicate SEM.