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Retinal βgal expression contributed to the production of tolerance. (A) Arrβgal and control mice were enucleated and/or irradiated to eliminate the retina as a source of Ag, and to deplete existing Tregs. Five months later, all mice were immunized with βgal and the DTH response was analyzed by ear testing with βgal 7 days post-immunization. (B) ROSA26 and control mice were similarly treated and then ear tested. Results are given as the mean ± SD and P values (t test) are indicated.
