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. Author manuscript; available in PMC: 2010 Oct 2.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 18;387(4):671–675. doi: 10.1016/j.bbrc.2009.07.074

Fig. 3.

Fig. 3

Verification of BK/ApoA1 interactions using reciprocal coIP. (A) CoIP of BKα with ApoA1 (lane 1) and IP of BKα (lane 2) reveal prominent bands at ∼120 kDa, the expected weight of chick BKα. (B) Reciprocal coIP of ApoA1 with BKα (lane 1) and IP of ApoA1 alone (lane 2) reveal a prominent band at ∼28 kDa, the expected weight of ApoA1. The control for both panels consisted of mixing IgG beads and lysate in the absence of an antibody (lane 3). The 55 kDa band is heavy IgG, and occurs when either monoclonal or polyclonal antibodies are used to both precipitate proteins and probe the blots. “M” denotes the standard.