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. 2009 Aug 25;106(39):16698–16703. doi: 10.1073/pnas.0905245106

Fig. 4.

Fig. 4.

Generation of retinal pigment epithelium. (A) Photomicrograph of adherent cultures showing pigmented, hexagonal RPE-like cells. (B) Immunostaining revealing expression of Mitf within RPE-like cells, as well as the tight junction protein ZO-1. (C) FACS analysis demonstrating the percentage of all adherent cells expressing Mitf and Pax6 at day 40 of differentiation. (D) RT-PCR studies showing expression of genes associated with an RPE fate. (Scale bars, 100 μm.)