Fig. 5.

Generation of early photoreceptor phenotypes. (A) Immunocytochemical detection of cells expressing the photoreceptor-specific transcription factor Crx at 80 days of differentiation. (B and C) Expression of the photoreceptor protein recoverin (B) and the cone photoreceptor-specific protein opsin (C) among Crx-expressing cells at day 80. (D) RT-PCR demonstrated the stepwise acquisition of a cone photoreceptor fate from an eye field population. (Scale bars, 50 μm.) Blue stain in A is Hoechst nuclear dye.