Fig. 4.

Cell transfer systems to provoke arthritis and endocarditis. (A) Arthritis scores and ankle thickness measurements following transfer of bone marrow from K/BxN mice into irradiated B6 Rag-deficient (diamonds) or BxN Rag-deficient (triangles) mice (mean, SEM). For the B6-Rag recipients, the mice were killed due to wasting disease 6 weeks following transplantation. (B) Arthritis scores and ankle thickness measurements following transfer of K/B splenocytes (circles) or K/BxN splenocytes (squares) into BxN TCRα−/− mice (mean, SEM). (C) The maximal thickness of the mitral valves was determined for each mouse at the time of sacrifice. Endocarditis was defined as in Fig. 2A. (D) Anti-GPI IgG titers were determined by ELISA for each mouse at the time of sacrifice (6–8 weeks following splenocyte or bone marrow cell transfer, sera were diluted 1:1,000). For (C) and (D), closed symbols indicate mice with endocarditis, whereas open symbols indicate mice without endocarditis; for one mouse (open symbol with “x” in D), serum was available but histologic evaluation of the heart was not. Bars indicate mean values; P values for comparisons between the groups are provided (Student's t test).