Fig. 2.
Expression and purification of TAP-Drs2p. (A) Chromosomal organization of TAP-DRS2 and CDC50. (B) Schematic of TAP-Drs2p. His10, decahistidine moiety; ProA, protein A moiety. (C) Aliquots from a TAP-Drs2p purification from strain XZY60m (atp2Δ) were subject to SDS/PAGE, and the gel was stained with SimplyBlue. Lane 1, cell lysate; lane 2, proteins bound to the IgG beads; lane 3, proteins bound to the Ni-NTA beads; lane 4, final eluate. (D) Comparison of activity of TAP-Drs2p and Drs2p-TAP ATPase purified from strain XZY60m (atp2Δ) and XZY38b (atp2Δ), respectively.