Fig. 6.

Ectopic overexpression of HDAC6 attenuates sulforaphane-mediated AR and HDAC6 protein depletion, and HDAC6 siRNA recapitulates the findings seen with sulforaphane. (A) LNCaP cells were transfected with pCDNA3.1 or FLAG-HDAC6. Cells were then treated with either vehicle or 15 μM sulforaphane. Intensity values for bands by Western blot in the respective vehicle controls were set to 1. (B) LNCaP cells were transfected with siRNA to either the luciferase gene (si LUC) or HDAC6 gene (si HDAC6). Levels of protein expression by Western blot were quantified, and the bands from the HDAC6 siRNA samples were compared to the luciferase control samples from the same time point.