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. 2009 Oct 13;4(10):e7376. doi: 10.1371/journal.pone.0007376

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Sasvari et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The wheat germ translation assay was programmed with artificial capped p33 mRNA carrying a poly(A) tail in the presence of CPZ, QC and DMSO as control. The radiolabeled p33 product was analyzed on SDS-PAGE. Each experiment was repeated three times. DMSO sample (0.03%) was chosen as 100%. (B) Similar in vitro translation assay was programmed with the uncapped wt TBSV gRNA, which does not carry a poly(A) tail.