Figure 4. Role of NR2E3 DBD mutations in the dimerization process and in the interaction with CRX.

A) HEK293T cells were transiently transfected with 2 µg of vectors expressing the wild-type (WT) NR2E3 fused to renilla luciferase (NR2E3_WT-RLuc) and with 4 µg vector expressing mutants of NR2E3 fused to the GFP² (GFP²-NR2E3_MUT). Control condition with RLuc alone and GFP²-NR2E3_WT served to calculated the background BRET² ratio. Luminescence ratio was measured as described in material and methods, and sesults were expressed as % of GFP²-NR2E3_WT/NR2E3_WT-RLuc and as mean±SEM of 3 experiments, * p<0.0001 and # p<0.01. (see supplemental figure 4 for BRET² titration curve). B) HEK293T cells were transiently transfected with 2 µg of vectors expressing the CRX fused to renilla luciferase (Crx-RLuc) and with 4 µg of vector expressing mutants of NR2E3 fused to the GFP² (NR2E3_MUT-GFP²). Control condition with RLuc alone and GFP²-NR2E3_WT served to calculated the background BRET² ratio. Luminescence ratio was measured as described in material and methods, and sesults were expressed as % of NR2E3_WT-GFP²/Crx-RLuc and as mean±SEM of 2 experiments, *p<0.003. (see supplemental figure 5 for BRET² titration curve).