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. 2009 Oct 13;4(10):e7439. doi: 10.1371/journal.pone.0007439

Figure 5. Activation state of various signaling pathways, in the triple mutant BCR-ABL expressing cells, relative to wild type.

Figure 5

32D cell lines expressing either wild type or triple mutant BCR-ABL were lysed and the activation state of the indicated signaling pathways was detected by immunoblotting whole cell lysates with phosphospecific antibodies for each pathway. (A) phospho-STAT5; (B) phospho-MAPK; (C) phospho-MEK; (D) phospho-AKT. The levels of phosphorylation were normalized for the whole cell levels of the protein shown in the lower panel of each immunoblot. (A) STAT5; (B) MAPK; (C) MEK; (D) AKT. Phosphorylation levels are reported relative to wild type (upper panels). Data represents three independent experiments with a representative blot shown.