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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Anal Chem. 2009 Sep 15;81(18):7611–7617. doi: 10.1021/ac9009935

Figure 4.

Figure 4

Top: L126C GM2AP amino acid sequence with location of reported disulfide bonds and predicted cleavages for trypsin (blue: K,R), Glu C (dark green: E), and Asp N (orange: D). The location of the L126C amino acid substitution is shown in bright green. The HIS10-tag (light gray) is engineered to include a Factor Xa cleavage site. Bottom: Sequence coverage for the L126C-4MT GM2AP construct after trypsin digestion and nano-LC MS analysis. The blue sequence was identified by MASCOT; the red sequence was identified by hand, and corresponds to the peptides still containing disulfide bonds.