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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Neurobiol Dis. 2009 Apr;34(1):146–154. doi: 10.1016/j.nbd.2009.01.002

Figure 4. Steady-state expression of myelin proteins and cytosolic chaperones is enhanced by the dietary regimen.

Figure 4

A-B, Whole sciatic nerve lysates (20 μg/lane) from Wt and TrJ mice were probed with the indicated anti-myelin protein (P0, PMP22 and MBP), or anti-heat shock protein (Hsp) antibodies. GAPDH is shown as a protein loading control. Molecular mass in kDa, on left. C, On longitudinal sections of sciatic nerves from intermittently fed neuropathic animals, MBP-positive myelin internodes are prominent in IF mice (arrows). Co-staining the sections with anti-HSC70 antibody reveals increased expression of this cytosolic chaperone within Schwann cells (arrows). Nuclei are stained with Hoechst dye (blue). Samples from Wt mice are shown in the insets for comparison. Micron bar, 20 μm.