Skip to main content
. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Neurobiol Dis. 2009 May;34(2):279–290. doi: 10.1016/j.nbd.2009.01.016

Figure 8.

Figure 8

Intraperitoneal rotenone results in the loss of nigral tyrosine hydroxylase- (TH) positive neurons in middle-aged rats. Tyrosine hydroxylase immunohistochemistry (A-I) in control (A-C), and rotenone-treated rats [2.75 mg/kg/day (D-F) or 3.0 mg/kg/day (G-I)]. Low magnification images show overall cell loss and pruning of processes (A,D,G) (bar = 500 μm), medium magnification images in dorsolateral and ventral regions of nigra show cell loss, morphological changes, and loss of processes in these particularly vulnerable areas (bar = 50 μm). Unbiased stereological cell counts (J,K) show quantitative measurement of cell loss. ANOVA with Tukey's post hoc test indicated that both rotenone doses caused significant loss of dopamine neurons. Because no differences were found between animals treated at 2.75 or 3.0 mg/kg/day (J), these groups were merged and subjected to a student's t-test (K). **p<0.01; ***p<0.001 compared to control.