Figure 1. PN increases phosphorylation of p38, MKK3/6 and ATF-2.
IEC-6 cells were exposed to 50 M PN and DPN for the indicated times. Naïve (untreated cells) are indicated by 0min. Levels of phospho-p38, phospho-MKK3/6, phospho-ATF-2, total p38, total MMK3 and total ATF-2 were determined by Western blotting. UV, cells exposed to 50mJ/cm2 UVC and allowed to recover in growth medium for 1h (positive control for p38 activation). Data shown are representative of at least 3 independent experiments.