FIGURE 1.

Effect of YafO on protein, DNA, and RNA synthesis. A, effect of YafO on [³⁵S]methionine incorporation in toluene-treated cells. E. coli BW25113 cells containing pBAD30-YafO were grown at 37 °C in glycerol-M9 medium. When the A₆₀₀ of the culture reached 0.4, arabinose was added to a final concentration of 0.2%. After incubation at 37 °C for 10 min, the cells were treated with toluene (11, 12). Using toluene-treated cells, ATP-dependent protein synthesis was carried out with [³⁵S]methionine as described previously (11). B, effect of YafO on [α-³²P]dCTP incorporation in toluene-treated cells (13). C, effect of YafO on [α-³²P]UTP incorporation in toluene-treated cells (14). D, growth curve of BW25113 cells containing pBAD30-YafO plasmid in the LB medium. Arabinose was added at 150 min as indicated by a solid arrow. E, effect of YafO on [³⁵S]methionine incorporation in vivo. [³⁵S]Methionine incorporation into E. coli BW25113 cells containing pBAD30-YafO was measured at various time points after YafO induction as indicated. F, SDS-PAGE analysis of in vivo protein synthesis after the induction of YafO. The same cultures as those in E were used.