Skip to main content
. 2009 Jul 17;284(38):25522–25531. doi: 10.1074/jbc.M109.036624

FIGURE 2.

FIGURE 2.

Effect of purified YafO-His6 on cell-free protein synthesis. A, purification of YafO-His6 and TF-YafN. Lane 1, purified YafO-His6; lane 2, purified TF-YafN. B, effect of YafO-His6 on MazG synthesis in a prokaryotic cell-free protein synthesis using E. coli T7 S30 extract system (Promega) and pET-11a-MazG as a template. Lane 1, without YafO-His6; lanes 2–4, 0.5, 2.0, and 8.0 μm YafO-His6 added, respectively; lanes 5 and 6, 8.0 μm YafO-His6 added with TF-YafN in the ratio to YafO-His6 of 0.46 and 1.73 respectively. C, effect of YafO-His6 and MazF-His6 on RNA stability. Total RNAs were mixed with different amount of protein and incubated at 37 °C for 15 min. The reaction products were analyzed by 1% agarose native gel electrophoresis followed by ethidium bromide staining. Lane 1, no protein was added; lane 2, 6.0 μm YafO-His6 was added; land 3, 15 μm YafO-His6 was added; lane 4, 0.08 μm MazF-His6 was added.