FIGURE 4.

In vivo identification of YafO cleavage sites in the chromosomally encoded ompA and ompF mRNAs. The ompA and ompF mRNAs were prepared from E. coli BW25113 cells containing pBAD30-YafO at various time points as indicated before and after the induction of YafO. The sequence ladders for ompA and ompF were obtained using pCR®2.1-TOPO®-ompA and pCR®2.1-TOPO®-ompF as template, respectively. The sequences around the major cleavage sites are shown to the right, and the major primer extension stop sites are indicated by the arrowheads. A, primer extension analysis of the ompA mRNA. B, primer extension analysis of the ompF mRNA.