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. 2009 Jul 17;284(38):25522–25531. doi: 10.1074/jbc.M109.036624

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Toeprinting of the ompA and ompF mRNAs after phenol extraction. A, primer extension analysis of the ompA mRNA after phenol extraction. The experiment was carried out in the same way as described for Fig. 5A except that reaction products in lanes 4, 6, and 8 were phenol-extracted to remove proteins before primer extension. B, toeprinting of the ompF mRNA after phenol extraction. The experiment was carried out in the same way as described for Fig. 5B except that reaction products in lanes 4, 6, and 8 were phenol-extracted to remove proteins before primer extension. Lane 1, without YafO-His₆; lane 2, 12.3 μm YafO-His₆; lane 3, 0.05 μm 70 S ribosomes with 12.3 μMYafO-His₆; lane 4, 0.05 μm 70 S ribosomes with 12.3 μm YafO-His₆; lane 5, 0.05 μm 70 S ribosomes with 1 μm tRNA_f^Met; lane 6, 0.05 μm 70 S ribosomes with 1 μm tRNA_f^Met; lane 7, 0.05 μm 70 S ribosomes, 1 μm tRNA_f^Met with 12.3 μm YafO-His₆; lane 8, 0.05 μm 70 S ribosomes, 1 μm tRNA_f^Met with 12.3 μm YafO-His₆.