FIGURE 9.

YafO associates with the 50 S ribosomal subunits. Ribosome profiles were analyzed by sucrose density gradient centrifugation as described under “Experimental Procedures.” A, polysome profiles of E. coli BW25113 containing pBAD30-YafO without YafO induction. B, polysome profiles of E. coli BW25113 containing pBAD30-YafO at 10 min after YafO induction. C, 70 S ribosomes of the same lysate used in B were disassociated into 50 and 30 S ribosomal subunits in 0.5 mm Mg²⁺. In B and C, Western blot analysis was carried out to detect YafO in each gradient fraction.