FIGURE 2.

Co-stimulating TLR2 and DC-SIGNR1 or with M. tb infection differentially modulates SOCS1 expression. A, DCs were stimulated with 0.5 μg/ml Pam₃Csk₄ alone (P) or 2.5 μg/ml manLAM (L) alone or co-stimulated with either equal concentrations of both (P = L) for indicated times. Alternatively, DCs were stimulated with 1 μg/ml Pam₃Csk₄ and 2.5 μg/ml manLAM (P > L), or with 0.5 μg/ml Pam₃Csk₄ and 5 μg/ml manLAM (P < L) for the indicated times. Post-incubation, cytoplasmic extracts were Western blotted for SOCS1. B, DCs were either stimulated with 1 μg/ml Pam₃Csk₄ alone (Pam) or 5 μg/ml manLAM alone or co-infected with 1 multiplicity of infection M. tb H37Rv for the indicated times. Post-incubation, cytoplasmic extracts were Western blotted for SOCS1 levels. Numbers below the SOCS1 blot indicate relative intensities of the bands. Data from one of three experiments are shown. C, IL-12p40 and IL-10 levels in the culture supernatants from B at 24-h post-stimulation. Data are the mean ± S.D. of three independent experiments.