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. 2009 Jul 17;284(38):25532–25541. doi: 10.1074/jbc.M109.006221

FIGURE 4.

FIGURE 4.

DC-SIGNR1 induces SOCS1 expression via Raf-1 and Syk and SOCS1 associates with DC-SIGNR1. A and B, SOCS1 levels in DCs stimulated with 1 μg/ml Pam3Csk4 (Pam) or 5 μg/ml manLAM for 24 h in the presence or absence of 5 μm and 10 μm GW5074 a Raf-1 inhibitor (Raf-1-inhib) or 5 μg/ml and 10 μg/ml piceatannol, a Syk inhibitor (Syk-inhib). For C and D, DCs from TLR4−/− mice were processed as in A and B. For E, either TLR2 or DC-SIGNR1 were immunoprecipitated from DCs following stimulation with 1 μg/ml Pam3Csk4 (Pam) or 5 μg/ml manLAM for the indicated times and Western blotted for SOCS1. Numbers below the SOCS1 blot indicate relative intensities of the bands. Data from one of three independent experiments are shown.