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. 2009 Jul 17;284(38):25532–25541. doi: 10.1074/jbc.M109.006221

FIGURE 5.

FIGURE 5.

Patients with active TB disease express high SOCS1 that is associated with DC-SIGN and regulated by Raf-1 and Syk. A, SOCS1 levels in PBMCs of healthy individual or patient with active TB disease and the same patient after 2 months of chemotherapy (Follow-up) following stimulation with 1 μg/ml Pam3Csk4 (P) or 5 μg/ml manLAM (L) for 24 h. One of eight similar blots is shown. For B, densitometric values of SOCS1 blots of all the healthy controls, patients, and follow-ups were employed to graphically represent SOCS1 levels in different cohorts. Bars represent mean ± S.D. of SOCS1 levels in each cohort irrespective of the treatment employed. C, PBMCs from a patient with active TB disease were simulated with 1 μg/ml Pam3Csk4 (Pam) or 5 μg/ml manLAM for 24 h. Cytoplasmic extracts were immunoprecipitated with either anti-TLR2 or anti-DC-SIGN antibody and Western blotted for SOCS1. D and E, SOCS1 levels in PBMCs of healthy individual stimulated with 1 μg/ml Pam3Csk4 (Pam) or 5 μg/ml manLAM for 24 h in the presence or absence of 5 μm and 10 μm GW5074 a Raf-1 inhibitor (Raf-1-inhib) or 5 μg/ml and 10 μg/ml piceatannol, a Syk inhibitor (Syk-inhib). One of three independent experiments is shown.