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. 2009 Jul 17;284(38):25532–25541. doi: 10.1074/jbc.M109.006221

FIGURE 8.

FIGURE 8.

DCs from M. tb-infected TLR2−/− mice show high SOCS1 expression upon DC-SIGNR1 stimulation and knockdown of SOCS1 kills intracellular M. tb. A, SOCS1 levels in splenic DCs from M. tb H37Rv-infected wild-type and TLR2−/− mice following stimulation with 1 μg/ml Pam3Csk4 (P) or 5 μg/ml manLAM (L) for 24 h. For B, wild-type and TLR2−/− mice were infected with M. tb H37Rv for 2 weeks followed by drug treatment. SOCS1 levels in splenic DCs were later monitored as in A. One of three experiments is shown. C and D, M. tb H37Rv colony forming units in mouse DCs (C) and human PBMCs (D) following knockdown of SOCS1 with specific siRNAs. MOCK represents no siRNA (vehicle control), while NS1 represents silencing with nonspecific control siRNAs procured from Santa Cruz Biotechnologies. NS2 to NS4 represent silencing with nonspecific siRNAs to P. falciparum genes Berghepain 1 (BP1), PF10_0348 (P. f DBLMSP), and PF10_0351, respectively, custom synthesized by Dharmacon. Data are the mean ± S.D. of three independent experiments.