FIGURE 2.

Adiponectin limits phosphorylation of IκB, JNK, and p38 in human macrophages stimulated with LPS. Cells were incubated without (left lanes) or with (right lanes) 10 μg/ml adiponectin for 18 h and subsequently stimulated with 5 ng/ml LPS for the indicated periods of time. Whole-cell lysates were fractionated by SDS-PAGE and immunoblotted with antibodies to phospho-IκB (A), phospho-JNK (B), or phospho-p38 (C). Actin, total JNK, and total p38 served as loading controls, respectively. Images are representative of three independent experiments on cells from different donors.