FIGURE 3.

Adiponectin inhibits TNFα- and IL6-induced signaling in human macrophages. Cells were incubated without (left lanes) or with (right lanes) 10 μg/ml adiponectin for 18 h and subsequently stimulated with 50 ng/ml TNFα (A–C) or 5 ng/ml IL6 (D) for the indicated periods of time. Whole-cell lysates were fractionated by SDS-PAGE and immunoblotted with antibodies to phospho-IκB (A), phospho-JNK (B), phospho-p38 (C), or phospho-STAT3 (D). Actin, total JNK, total p38, and total STAT3 served as loading controls, respectively.