Skip to main content
. 2009 Jul 18;284(38):25569–25575. doi: 10.1074/jbc.M109.019786

FIGURE 5.

FIGURE 5.

IL10 neutralization does not impair the actions of adiponectin in human macrophages stimulated with TNFα or IL6. A, cells were incubated with (lanes 3–6) or without (lanes 1 and 2) 10 μg/ml adiponectin for 18 h in the presence of anti-IL10 antibody (lane 5) or control IgG (lane 6) and subsequently stimulated with 50 ng/ml TNFα or left unstimulated for 10 min. Whole-cell lysates were fractionated by SDS-PAGE and immunoblotted with antibodies to phospho-IκB (top), phospho-JNK (middle), or phospho-p38 (bottom). Actin, total JNK, and total p38 served as loading controls, respectively. B, cells were incubated with (lanes 3–6) or without (lanes 1 and 2) 10 μg/ml adiponectin for 18 h in the presence of anti-IL10 antibody (lane 5) or control IgG (lane 6) and subsequently stimulated with 5 ng/ml IL6 or left unstimulated for 40 min. Whole-cell lysates were fractionated by SDS-PAGE and immunoblotted with antibodies to phospho-STAT3. Total STAT3 served as loading control.