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LT inhibits AP-1 DNA binding activity. Cells were treated with medium alone or medium containing LT, TNF, or both. Binding activity of AP-1 was determined by trans-binding ELISA as described under “Experimental Procedures.” Means ± S.E. for four separate experiments are shown. **, p < 0.01 versus medium; *, p < 0.05 versus medium; ††, p < 0.01 versus TNF. ABS, absorbance.
