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. 2009 Jul 16;284(38):25791–25803. doi: 10.1074/jbc.M109.033993

FIGURE 5.

FIGURE 5.

There was a diurnal expression of gga-mir-26a in the chicken retina. A, the top shows the sequence alignment of mature mir-26a among human (Hsa), mouse (Mmu), dog (Rno), chicken (Gga), and Drosophila (Dre). *, identical nucleotides. The lower panel shows the RT-PCR amplification of gga-mir-26a precursor from the chicken retina harvested at ZT 5 (day) and ZT 17 (night). β-Actin served as an internal control. B, there were diurnal expressions of mature gga-mir-26a (mir-26a) and mRNAs of Bmal1 and L-VGCCα1C in the chicken retina. Upper left, gga-mir-26a reached its highest levels at ZT 4, which was significantly different from the other five time points (n = 5 for each time point; *, p < 0.05). Upper right, the mRNA levels of Bmal1 at ZT 8 and 12 are significantly different from ZT 0, 4, 16, and 20 (n = 6 for each time point; *, p < 0.05). Lower left, the mRNA levels of L-VGCCα1C at ZT 12 and 16 are significantly higher than ZT 0, 4, 8, and 20 (n = 6 for each time point; *, p < 0.05).