TABLE 1.

List of primers used to amplify the gga-mir-26a precursor and promoter (and mutations), gga-mir-124a precursor, and L-VGCCα1C 3′-UTR (and mutation)

PCR fragment	Primer	Sequence
gga-mir-26a precursor	Forward	5′-GGATCCATGTTAGCACGTCTGCTGTG-3′
	Reverse	5′-AAGCTTTGCCCGTGACATCTCAAGCTTCT-3′
gga-mir-124a precursor	Forward	5′-AGGCTCTGCCTCTCCGTGTTCACAG-3′
	Reverse	5′-AATGCCGCCTGGAGGATCCGCTCTT-3′
Chicken L-VGCCα1C 3′-UTR fragment	Forward	5′-ACTAGATTACAATCATAAGTCGTGTTGGC-3′
	Reverse	5′-TCTAGACTCAAACAAACTGAATTCGATTC-3′
Point mutation of chicken L-VGCCα1C 3′-UTR fragment	Forward	5′-GATGTTTCTGTTGAAGAAACCGTTATACTaaAATTCAGGTCAGTTTCGG-3′
	Reverse	5′-CCGAAACTGACCTGAATTttAGTATAACGGTTTCTTCAACAGAAACATC-3′
2-kb gga-mir-26a promoter	Forward	5′-TATGAGCTCTTAACCTGTGATGTTCACTAGAAC-3′
	Reverse	5′-ACACTCGAGGTAACAGGATTAAGGAGTTTTGGC-3′
−150–+1(Δ−2kb−150) fragment of gga-mir-26a promoter	Forward	5′-TATGCTAGCTTGCTTGATATTAGTTGTTGAA-3′
	Reverse	5′-ACACTCGAGGTAACAGGATTAAGGAGTTTTGGC-3′
−2kb−300(Δ−300–+1) fragment of gga-mir-26a promoter	Forward	5′-TATGAGCTCTTAACCTGTGATGTTCACTAGAAC-3′
	Reverse	5′-AATGCTAGCTGCAGTGGTGGACACGGTTTAGC-3′