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Assay of the V. cholerae late acyltransferases in the presence of phosphorylated Kdo-lipid IVA (P-Kdo-lipid IVA), or Kdo2-lipid IVA. E. coli MKV15 (DE3) membranes expressing the V. cholerae late acyltransferases (Vc0212, Vc0213, or Vc1577) were assayed for activity at 0.01 mg/ml for 1 h at 30 °C with a mixture of acyl-ACPs (C12:0, C14:0, C16:0, and C18:0) and either P-Kdo-[4′-32P]lipid IVA (A) or Kdo2-[4′-32P]lipid IVA (B).
