FIGURE 3.

Glutamine-induced currents in SNAT3 mutant N76D. Oocytes were injected with 20 ng of cRNA encoding SNAT3 mutant N76D, and glutamine-induced (10 mm) currents were analyzed after 3–6 days of expression at pH 7.4 and pH 8.4 and under different ionic conditions as indicated. Sodium ions were replaced by NMDG⁺, and chloride ions were replaced by gluconate⁻. Typical recordings are depicted from oocytes expressing SNAT3 mutant N76D (A); the corresponding current-voltage relationship for SNAT3 mutant N76D is depicted in B (n = 8). The responses to voltage jumps (A) at the indicated positions (a, b, c, and d) were converted into I/V plots (B) and labeled accordingly. NMDG, N-methyl-d-glucamine.