TABLE 2.

Changes of intracellular pH induced by substrates in SNAT3 and its mutants

The change of [H⁺]/min was recorded immediately after the addition of 10 mm glutamine for SNAT3 wild type and its mutants at pH 8.4, and the S.E. was calculated (n = 12 experiments). ND, not detectable; NI, non-injected oocytes.

	SNAT3	N76D	N76E	N76Q	N76H	NI
	nm H+/min	nm H+/min	nm H+/min	nm H+/min	nm H+/min	nm H+/min
100 mm Na+	−33.3 ± 11	−8.5 ± 2.7	−12.4 ± 3.6	−9.6 ± 2.9	−3.1 ± 0.4	0.015 ± 0.015
0 Na+	−9.1 ± 5	−4.7 ± 1.5	−2.2 ± 0.6	−0.9 ± 0.4	−0.63 ± 0.3	ND