TABLE 2.

Phosphokinase profile of the three MEF strains

MEF were stimulated with 1 ng/ml IL-1β for 30 min, and then the lysates were subjected to protein array analysis and densitometry. The table shows mean ± S.E. -fold changes in kinase activity in the two knockout strains compared with WT MEF, which is set at 1; n = 2. The top comprises kinases that exhibit increased activation in IL-18Rα ko and/or decreased activation in IL-18 ko compared with WT. The middle shows kinases the activity of which is increased in IL-18 ko MEF, and the bottom shows pathways with little change. Underlining, kinase activity decreased by more than −33%; normal type, change between −33 and +33%; italic type, increase between 33 and 67%; boldface type, increase of >67%.

	IL-18 ko	IL-18Rα ko
Activation of IL-18Rα ko > IL-18 ko
Akt1/PKBα	−36.5 ± 3.3	122.6 ± 28.4
Akt2/PKBβ	2.1 ± 13.7	52.6 ± 5.3
Akt pan	−23.7 ± 6.7	89.7 ± 21.7
ERK1-p44	−0.9 ± 15.4	68.7 ± 17.7
ERK2-p42	−5.8 ± 18.1	60.1 ± 1.7
p38α	15.5 ± 17.3	76.3 ± 20.4
p38β	1.2 ± 28.1	88.1 ± 19.3
p38γ	−12 ± 3	31.1 ± 17.1
GSK3α and -β	−31.5 ± 12.3	19.9 ± 4.3
GSK3β	−46.3 ± 9.4	6.2 ± 5.8
p70 S6 kinase	−9.2 ± 2.1	36.3 ± 10.5
Activation of IL-18Rα ko < IL-18 ko
p38δ	56.0 ± 15.4	−5.4 ± 3.8
MSK2/RSKβ	40.6 ± 10.3	−10.6 ± 9
Little change in activation
Akt3/PKBγ	−11.7 ± 20.5	31 ± 23.4
JNK1	11.6 ± 4	13.3 ± 13.2
JNK2	0.5 ± 3.7	32.6 ± 4.4
JNK3	16.3 ± 6	38.0 ± 14.4
JNK pan	12.3 ± 9.9	29.7 ± 22
HSP27	−3.1 ± 3.6	6.1 ± 1.3
RSK1	−6.3 ± 12.4	28.2 ± 0.4
RSK2	4.2 ± 21.5	−0.6 ± 16.5