FIGURE 6.

Activation of CAR prevented fatty acid overloading and incomplete β-oxidation in the skeletal muscle. A and B, liver (A) and skeletal muscle (B) mRNA expression of genes involved in β-oxidation. Male C57BL/6J mice were HFD-fed and TOPOBOP-treated for 8 weeks. n = 4 for each group. C and D, complete oxidation of [¹⁴C]oleate to CO₂ (C) or incomplete oxidation of [¹⁴C]oleate to acid-soluble metabolite (ASM) (D) in mitochondria isolated from the gastrocnemius muscle, in the absence or presence of pyruvate. Mice are the same as those described in A and B. *, p < 0.05; **, p < 0.01; NS, statistically not significant (p > 0.05) compared with vehicle group, or the comparisons are labeled.