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(a) CD spectra of pIIGp2(552–650) recorded at 20°C and 95°C, and after one cycle of heating to 95°C and cooling down to 20°C (refolded). (b) Thermal denaturation curve of pIIGp2(552–650) recorded at 222 nm with a scan rate of 1°C per min. (c) Denaturation of pIIGp2(552–650) in guanidine hydrochloride. The CD signal was monitored at 222 nm.
