TABLE 2.

Effect of divalent cations on the binding affinity of human Ago2 for various antisense oligonucleotides

The dissociation constants (K_d) for human Ago2 containing the various antisense oligonucleotide configurations in the presence of either Mg²⁺ or Mn²⁺ were determined as described under “Materials and Methods.” Sequences of the unmodified 19-nucleotide antisense RNA (19-as) and antisense RNAs containing 5′-hydroxyl (OH), abasic residue (p/a), or deoxyribonucleotides substitutions (_dN). The errors reported for the Ago2 binding affinities are based on three trials.