FIGURE 2.

Time-dependent PDGF receptor (PDGFR) autophosphorylation under mechanical strain. The adherent cells were conditioned overnight, and then stretched at 8% for the time indicated. Immunoblot analyses were performed using antibody reactive with PDGFR-α and -β, phosphorylated PDGFR-α (p-PDGFR-α) and -β, or GAPDH (as a control). The phosphorylated profiles of the PDGFR-α and -β were aligned (A, a representative of three independent experiments) and the time-dependent receptor phosphorylations (p-PDGFR-α) were traced up to 30 min and analyzed (B). Bands were visualized by an ECL method and quantified by a densitometer. Data were summarized, plotted, and normalized independently for comparison as -fold of increases for the p-PDGFR-α (A and B) or p-PDGFR-β (A). *, p < 0.05; ^#, p < 0.01, as compared with the unstrained cells. This was a result of three sets of independent experiments.