FIGURE 8.
Synthetic GaTx2 inhibits ClC-2 by slowing channel opening and does not affect single-channel conductance. A, all points amplitude histograms in the absence (left panel) and presence (right panel) of 2 nm GaTx2. B, representative single channel trace of ClC-2 with and without 20 pm synthetic toxin at VM = −100 mV. The traces shown begin with the step to −100 mV. C, expanded single channel trace of a ClC-2 burst in the absence and presence of 20 pm GaTx2, from the records shown in B. D, comparison of channel activity (as NPO/N) in the absence and presence of 20 pm GaTx2. E, comparison of latency to opening of the first double-barreled burst after stepping from 0 to −100 mV, from records such as those shown in B in the absence and presence of 20 pm GaTx2.