FIGURE 9.
GaTx2 cannot inhibit open ClC-2 channels. ClC-2 channels were studied using outside-out macropatches activated by a voltage step to VM = −100 mV. Channel activity was allowed to reach steady state. The patches were then exposed to either 2 nm GaTx2 (A) or 1 mm DPC (B). Summary data shown in C and D indicate that although GaTx2 was unable to inhibit ClC-2 steady state currents from open channels (n = 5) (C), DPC was able to inhibit very well (n = 9) (D). The inset in A, on the other hand, shows that if GaTx2 was applied to channels during the activation phase in this protocol, then currents were inhibited.