FIGURE 7.

Tumors formed by syndecan-1 knockdown cells have a poorly developed vasculature and decreased levels of human VEGF. A, immunohistochemistry of xenograft tumor tissues from tumors formed by control or syn1A knockdown cells and stained with antibody to CD34 (brown) and counterstained with hematoxylin (blue) (original magnification, ×200). Inset, negative control. Bar graphs, quantification of microvessel density and average vessel length. The data are the means ± S.E. B, immunohistochemistry for human VEGF (brown) in xenograft tumor sections counterstained with hematoxylin (blue). Inset, antibody negative control (original magnification, ×400). C, medium conditioned by myeloma cells for 48 h was collected and levels of human VEGF assessed by ELISA. The data are the means ± S.E. (n = 3).