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. 2009 Jul 24;284(38):26207–26215. doi: 10.1074/jbc.M109.029249

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Correlations between ligand degradation by D6-expressing cells. Ligand degradation efficiency, calculated as the percentage of chemokine removed from the supernatant after 3 h of incubation with CHO-K1/hD6 cells, has been correlated with the binding affinities (K_i) measured in competition binding with ¹²⁵I-CCL4 on CHO-K1/hD6 (A), with the percentage of up-regulation of D6 membrane expression after 1 h of chemokine stimulation (B), or with the percentage of ¹²⁵I-CCL4 dissociation after 3 h of incubation with unlabeled chemokines (C). The lines represent linear regression calculated for the indicated ligands.