TABLE 1.
Binding constants of different chemokines and their processed isoforms for human and murine D6
Heterologous dissociation data obtained using the radiolabeled ligands 125I-labeled human CCL4 or CCL2 (where indicated) were analyzed using the one-site competitive binding equation to estimate the IC50 value, from which the Ki value was then calculated according to the Cheng-Prusoff equation.
| Ligand |
Ki ± S.E. |
|
|---|---|---|
| Human D6 | Murine D6 | |
| nm | ||
| CCL2 | 2.2 ± 0.11a | NDb |
| CCL3 | 17.25 ± 1.48 | ND |
| CCL3(5–68) | 4.68 ± 0.97 | ND |
| CCL3L1 | 1.21 ± 1.48 | ND |
| CCL4 | 1.46 ± 1.11 | 0.16 ± 0.004 |
| CCL8 | 10 ± 0.17a | ND |
| CCL8(6–75) | 20 ± 0.3a | ND |
| CCL14(1–74) | 23.40 ± 3.34 | 6.4 ± 2.83 |
| CCL14(9–74) | 1.53 ± 0.25 | 0.75 ± 0.39 |
| CCL14(11–74) | 6.5 ± 1.2 | ND |
| CCL17 | 17.25 ± 5.02 | ND |
| CCL22 | 0.48 ± 0.21 | ND |
a Data obtained using 125I-labeled human CCL2.
b ND, not detected.