Fig. 5.

14 amino acid region confers intracellular localization to TLR4. A, HeLa cells were transfected in 6 well plates with the indicated GFP-tagged chimeras and 24 hours later harvested for GFP immunoprecipitation and glycosylation analysis. Arrowhead indicates mature glycoform. The second and third lanes were separated by the molecular weight marker in the original gel. B, HEK293 cells were transfected with MD2 plus 1.5 or 150 ng/well of TLR4, TLR4-9 or TLR4-9-4. Cells were stimulated with 100 ng/ml LPS and NF-κB activity was measured 24 hours later.