Vesicle motif targets CpG DNA in an early endosome. A, HeLa cells were transfected with TacTIR9Δ898 and incubated with Cy-3 labeled CpG DNA for 1 hour prior to fixation and staining. Tac (green), CpG DNA (red). B, TacTIR9Δ898 transfected HeLa cells were stained for Tac (green) and LAMP-1 (red). C, HeLa cells were co-transfected with TacTIR9Δ898 and Rab5-GFP (green), an early endosome marker, and stained for Tac (red). Single slice images (0.8 μM) were collected on a Zeiss LSM510 Meta confocal microscope with a 63× objective. Sections of overlays were magnified for better visualization of co-localization (insets).