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. Author manuscript; available in PMC: 2009 Oct 6.
Published in final edited form as: J Cell Biochem. 2009 May 15;107(2):214–223. doi: 10.1002/jcb.22117

Figure 5. DNA Synthesis in Zygotes from Pronuclear Transfer.

Figure 5

(A–F) Zygotes were created by transferring S phase pronuclei into S phase parthenotes, as in Fig. 4A (arrow in D–F indicates pronuclear fragment that was transferred in this oocyte that continued to replicate); (G–L) G1 pronuclei into S phase ooplasm in parthenotes, as in Fig. 4B; (M–R) or by transferring S phase pronuclei into G1 ooplasm in parthenotes, as in Fig. 4C. The zygotes were then incubated in BrdU for 30, 3 hr or 4 h, as indicated on the left hand panel, then fixed and stained for BrdU incorporation and DNA with propidium iodide. Phase contrast, BrdU staining and propidium iodide staining are shown, as indicated. Bar = 20 µm.