Table 1. Mouse Pronuclei Initiate DNA Synthesis Synchronously.
Exp. | Hr after Fertilization1 |
No. (%) Oocytes with BrdU Positive Pronuclei (PN) |
||
---|---|---|---|---|
0 PN | 1 PN | 2 PN | ||
ICSI | 5 | 17 (94) | 0 | 1 (6) |
6 | 9 (32) | 0 | 19 (68) | |
7 | 4 (19) | 0 | 17 (82) | |
8 | 0 | 0 | 34 (100) | |
IVF | 5 | 29 (83) | 1 (3) | 5 (14) |
6 | 5 (19) | 0 | 21 (81) | |
7 | 1 (6) | 0 | 17 (94) | |
8 | 1 (6) | 0 | 15 (94) |
Fertilization = approximate moment of sperm-oocyte fusion; in IVF this was 30 min after initiation of gamete co-incubation and in ICSI it was the end of ≤ 10 min long injection of one group of oocytes.