Table 2. Pronuclear Microinjection Experiments.
Exp. ID | Age of Trans’d. PN (hr) |
Age of Oocyte (hr) |
No. Oocytes |
BrdU Positive | |||
---|---|---|---|---|---|---|---|
no PN | 1 PN (Trans’d., ♂) |
1 PN (Acceptor, ♀) |
2 PN (♂ + ♀) | ||||
Controls | |||||||
ICSI | NA | 7.0 | 22 | 3 (13.6%) | 0 | 1 (4.5%) | 18 (81.8%) |
P1 | NA | 4.5 | 20 | 20 (100%) | NA | 0 | NA |
P2 | NA | 7.0 | 28 | 1 (3.6%) | NA | 27 (96.4%) | NA |
Tested groups | |||||||
3 | 7 | 7 | 47 | 0 | 0 | 10 (21%) | 37 (79%) |
4 | 4.5 | 7 | 23 | 0 | 0 | 23 (100%) | 0 |
5 | 4.5 | 7 | 27 | 0 | 0 | 2 (7.4%) | 25 (92.6%) |
6 | 7 | 3 | 37 | 13 (35%) | 24 (65%) | 0 | 0 |
7 | 7 | 3 | 21 | 1 (5%) | 1 (5%) | 0 | 19 (90%) |